Protein phosphorylation in human neutrophils (PMN) induced by phorbol myristate acetate (PMA) and formyl-Met-Leu-Phe (fMLP)

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6829707

Caldwell, S E; McPhail, L C; Bass, D A; McCall, C E

Phosphorylation may regulate PMN responses to stimulation by PMA or fMLP. They examined differences in protein phosphorylation induced by either agent using cellular subfractionation. PMN were loaded with /sup 32/P, and stimulated with 100 ..mu..g/ml PMA for 20 min, or 10/sup -6/M fMLP + 10/sup -5/M cytochalasin B for 30 sec - conditions identical to those required for optimal stimulation of the respiratory burst by each agent. PMN were sonicated, and ultracentrifuged to yield cytosolic and particulate fractions. Protein samples were analyzed by SDS-gel electrophoresis, silver staining, and autoradiography. 43 phosphoproteins were identified in cytosolic fractions, and 40 in particulate fractions. In cytosolic fractions, as many as 22 phosphoproteins were enhanced by PMA stimulation and 12 with fMLP treatment. A cluster of phosphoproteins, approx. 38-43 kd, was enhanced by PMA, but not by fMLP, stimulation. As many as 6 particulate fraction proteins were more heavily phosphorylated with PMA treatment, and 11 with fMLP stimulation. In particulate fractions, a 26 kd phosphoprotein appeared upon PMA treatment, but not with fMLP. Characterization of the phosphoproteins which differ between the two treatments should aid in understanding the differential effects of PMA and fMLP on PMN function.

• Research Organization: Wake Forest Univ. Medical Center, Winston-Salem, NC
• OSTI ID: 6829707
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ESTERS
FUNCTIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MAN
MATERIALS
NEUTROPHILS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PRIMATES
PROTEINS
RADIOISOTOPES
VERTEBRATES