Identification of 5[prime]-flanking regions affecting the expression of the human decay accelerating factor gene and their role in tissue-specific expression
Journal Article
·
· Journal of Immunology; (United States)
OSTI ID:6827110
- Washington Univ., St. Louis, MO (United States)
Decay accelerating factor (DAF) is a complement regulatory protein that protects host tissue from complement-mediated damage by preventing the assembly and/or promoting the dissociation of C3 an C5 convertases. To identify and analyze the DNA sequence elements responsible for controlling DAF expression, the 5[prime]-flanking region of the human DAF gene was cloned. Sequencing of 880 nucleotides upstream from the ATG codon revealed the absence of classic TATA or CAAT boxes. RNase protection and primer extension assays revealed a series of transcription start sites in a 10 nucleotide region located 86 nucleotides upstream from the ATG (the first of these start sites is numbered +1). Using HeLa, K562, EBV, and Molt 4 cells, DAF mRNA and protein were analyzed by Northern and Western blot. The DAF mRNA and protein levels roughly correlated, suggesting transcriptional control of gene expression. The identification of transcription start sites and enhancer regions in the DAF gene will be important for studies of the mechanisms whereby cytokines and other factors may modulate DAF expression. 55 refs., 7 figs.
- OSTI ID:
- 6827110
- Journal Information:
- Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 150:1; ISSN JOIMA3; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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