Genomic structure and chromosomal mapping of the murine CD40 gene

Journal Article · Tue Dec 15 04:00:00 EST 1992 · Journal of Immunology; (United States)

OSTI ID:6827080

Grimaldi, J C; Chang, R; Howard, M; Cockayne, D A ^[1]; Torres, R; Clark, E A ^[2]; Kozak, C A ^[3]

---

1. DNAX Research Inst. of Molecular and Cellular Biology, Palo Alto, CA (United States)
2. Univ. of Washington, Seattle (United States)
3. National Institutes of Health, Bethesda, MD (United States)

The B cell-associated surface molecule, CD40, is likely to play a central role in the expansion of Ag-stimulated B cells, and their interaction with activated Th cells. In this study the authors have isolated genomic clones of murine CD40 from a mouse liver genomic DNA library. Comparison with the murine CD40 cDNA sequence revealed the presence of nine exons that together contain the entire murine CD40 coding region, and span approximately 16.3 kb of genomic DNA. The intron/exon structure of the CD40 gene resembles that of the low affinity nerve growth factor receptor gene, a close homolog of both human and murine CD40. In both cases the functional domains of the receptor molecules are separated onto different exons throughout the genes. Southern blot analysis demonstrated that murine CD40 is a single copy gene that maps in the distal region of mouse chromosome 2. 58 refs., 4 figs., 1 tab.

• OSTI ID: 6827080
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 149:12; ISSN 0022-1767; ISSN JOIMA3
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
DNA SEQUENCING
EXPERIMENTAL DATA
GENES
GENETIC MAPPING
INFORMATION
LEUKOCYTES
LYMPHOCYTES
MAPPING
MATERIALS
MEMBRANE PROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS