Nutrient resuscitation and growth of starved cells in sandstone cores: a novel approach to enhanced oil recovery. [Klebsiella pneumoniae]
Klebsiella pneumoniae, which was reduced in size (0.25 by 0.5 ..mu..m) by carbon deprivation, was injected into a series of sandstone cores and subjected to separate treatments. Scanning electron microscopy of 400-mD cores showed these small starved cells in nearly every core section. The cells were a mixture of small rods and cocci with little or no biofilm production. Continuous or dose stimulation with sodium citrate allowed the cells to grow throughout the sandstone and completely plug the length of the core. The resuscitated cells were larger than the starved cells (up to 1.7 ..mu..m) and were encased in glycocalyx. Scanning electron microscopic results of resuscitation in situ with half-strength brain heart infusion broth showed that a shallow skin plug of cells formed at the core inlet and that fewer cells were located in the lower sections. Starved cells also penetrated 200-mD cores and were successfully resuscitated in situ with sodium citrate, so that the entire core was plugged. Nutrient resuscitation of injected starved cells to produce full-size cells which grow and block the rock pores may be successfully applied to selective plugging and may effectively increase oil recovery.
- Research Organization:
- Univ. of Calgary, Alberta (Canada)
- OSTI ID:
- 6818476
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 54:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DRILL CORES
PLUGGING
KLEBSIELLA
GROWTH
MICROBIAL EOR
CITRATES
NUTRITION
OIL WELLS
PERMEABILITY
SODIUM COMPOUNDS
WATERFLOODING
ALKALI METAL COMPOUNDS
BACTERIA
CARBOXYLIC ACID SALTS
ENHANCED RECOVERY
FLUID INJECTION
MICROORGANISMS
RECOVERY
WELLS
020300* - Petroleum- Drilling & Production
550500 - Metabolism