Effects of. beta. -arabinofuranosyladenine on the growth and repair of potentially lethal damage in Ehrlich ascites tumor cells

Journal Article · Mon Sep 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575335· OSTI ID:6818022

Iliakis, G

..beta..-D-Arabinofuranosyladenine (..beta..-araA) inhibit the growth of Ehrlich ascites tumor cells by selective inhibition of DNA polymerases. RNA and protein synthesis are not significantly affected. Addition of ..beta..-araA to the cells after irradiation resulted in a concentration-dependent decrease in survival, presumably due to the inhibition of the repair of potentially lethal damage. Since ..beta..-araA selectively inhibits DNA polymerases it is suggested that repair of potentially lethal damage involves steps at the DNA level which require some polymerization. These repair steps take place in the DNA with a velocity comparable to that of the repair of potentially lethal damage. The inhibition of the repair of potentially lethal damage by ..beta..-araA was modified by the addition of deoxyadenosine; this supports the finding that ..beta..-araA acts competitively against dATP at the molecular level. The inhibition of the repair of potentially lethal damage by ..beta..-araA, which is partly reversible, resulted in a concentration-dependent modification of the survival curve. At low concentrations of ..beta..-araA a dose-modifying decrease in survival was observed. At higher concentrations (more than 12 ..mu..M) the decrease in survival resulted in a decrease of the shoulder width of the survival curve. Eventually an exponential curve was obtained. We suggest therefore that the shoulder of the survival curve results from some repair or potentially lethal damage. Preliminary information has been obtained on the time course of this repair.

• Research Organization: Gesellschaft fuer Strahlen und Umweltforschung, Frankfurt am Main, Germany
• OSTI ID: 6818022
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 83:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
ANIMAL CELLS
ASCITES TUMOR CELLS
ATP
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CHEMICAL REACTIONS
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYMES
EXPERIMENTAL DATA
GROWTH
HETEROCYCLIC COMPOUNDS
INFORMATION
INHIBITION
INJURIES
IONIZING RADIATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERASES
POLYMERIZATION
PROTEINS
PURINES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
RNA
SURVIVAL CURVES
SYNTHESIS
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS
X RADIATION