Characterization of recombinant bacteriophages containing mosquito ribosomal RNA genes

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6810280

Park, Y J

A family of nine recombinant bacteriophages containing rRNA genes from cultured cells of the mosquito, Aedes albopictus, has been isolated by screening two different genomic DNA libraries - Charon 30 and EMBL 3 using {sup 32}P-labeled 18S and 28S rRNA as probes. These nine recombinant bacteriophages were characterized by restriction mapping, Southern blotting, and S1 nuclease analysis. The 18S rRNA coding region contains an evolutionarily conserved EcoRI site near the 3{prime}-end, and measures 1800 bp. The 28S rRNA genes were divided into {alpha} and {beta} coding regions measuring 1750 bp and 2000 bp, respectively. The gap between these two regions measures about 340 bp. No insertion sequences were found in the rRNA coding regions. The entire rDNA repeat unit had a minimum length of 15.6 kb, including a nontranscribed spacer region. The non-transcribed spacer region of cloned A. albopictus rDNA contained a common series of seven PvuI sites within a 1250 bp region upstream of the 18S rRNA coding region, and a proportion of this region also showed heterogeneity both in the length and in the restriction sites.

• Research Organization: Rutgers-the State Univ., New Brunswick, NJ (USA)
• OSTI ID: 6810280
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
14 SOLAR ENERGY
BACTERIOPHAGES
CHEMICAL COMPOSITION
RIBOSOMAL RNA
GENES
DNA
GENETIC ENGINEERING
GENETIC MAPPING
MOSQUITOES
PHOSPHORUS 32
TRACER TECHNIQUES
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIPTERA
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
VIRUSES
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