Increased slow transport in axons of regenerating newt limbs after a nerve conditioning lesion made prior to amputation
Thesis/Dissertation
·
OSTI ID:6810207
The first part of this study shows that axonal density is constant in the limb stump of the next proximal to the area of traumatic nerve degeneration caused by limb amputation. The results of the second part of this work reveal that a nerve conditioning lesion made two weeks prior to amputation is associated with accelerated limb regeneration and that this accelerated limb regeneration is accompanied by an earlier arrival of axons. This is the first demonstration of naturally occurring limb regeneration being enhanced. In this study SCb cytoskeletal proteins were identified and measured using SDS-PAGE and liquid scintillation counting. Proteins were measured at 7, 14, 21, and 28 days after {sup 35}S-methionine injection and the normal rate of SCb transport determined to be 0.19 mm/day. A single axotomy does not enhance the rate of SCb transport but does increase the amount of labeled SCb proteins that are transported. When a conditioning lesion is employed prior to limb amputation and SCb proteins are measured at 7, 14, and 21 days after injection, there is a twofold acceleration in the rate of SCb transport and an increase in the amount of SCb proteins transported in conditioned axons.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA)
- OSTI ID:
- 6810207
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOSYNTHESIS
BODY
BODY AREAS
CARBOXYLIC ACIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNCTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIMBS
LIPOTROPIC FACTORS
MEMBRANE TRANSPORT
METHIONINE
NERVE CELLS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RECOVERY
SALAMANDERS
SCINTILLATION COUNTING
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOSYNTHESIS
BODY
BODY AREAS
CARBOXYLIC ACIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNCTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIMBS
LIPOTROPIC FACTORS
MEMBRANE TRANSPORT
METHIONINE
NERVE CELLS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RECOVERY
SALAMANDERS
SCINTILLATION COUNTING
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES