Comparison of the fates of ingested leucine and ingested 2-ketoisocaproate in rats

Journal Article · Tue May 01 04:00:00 EDT 1990 · American Journal of Clinical Nutrition; (USA)

OSTI ID:6809808

Imura, K; Walser, M ^[1]

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1. Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)

We previously reported that the ratio, R, of 14C to 3H in the leucine of whole body protein, measured 6 h after ingestion of (3H)leucine and (1-14C)2-ketoisocaproate is equal to ratio of the dose of leucine to the dose of 2-ketoisocaproate (KIC) (on a leucine-free diet) required to achieve the same rate of growth. To determine whether R is dependent on the interval between injection and sampling, R was measured at intervals in purified whole body protein after oral injection of these isotopes in groups of rats; it was constant from 1 h onward for 1 wk, averaging 0.64 +/- 0.01 (means +/- SEM). Thus, the extent of incorporation into the leucine of whole body protein of ingested KIC remains close to 64% of the incorporation of ingested leucine administered as such simultaneously, from 1 h onward for at least 1 wk.

• OSTI ID: 6809808
• Journal Information: American Journal of Clinical Nutrition; (USA), Journal Name: American Journal of Clinical Nutrition; (USA) Vol. 51:5; ISSN 0002-9165; ISSN AJCNA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMINO ACIDS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HYDROGEN COMPOUNDS
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
STOMACH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES