FMLP provokes coronary vasoconstriction and myocardial ischemia in rabbits

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6808793

Gillespie, M N; Booth, D C; Friedman, B J; Cunningham, M R; Jay, M; De Maria, A N ^[1]

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1. Univ. of Kentucky, Lexington (USA)

Recent pathological studies of coronary arteries from humans with suspected coronary spasm have revealed an augmented intramural burden of inflammatory cells. To test the hypothesis than inappropriate activation of inflammatory cells participates in the evolution of coronary vasospasm, the present experiment employed a newly developed coronary arteriographic technique for use in pentobarbital-anesthetized rabbits to evaluate the coronary vasomotor actions of the nonselective inflammatory cell stimulant, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). In 10 of 10 animals, selective left intracoronary injection of 200 ng fMLP evoked profound left coronary narrowing accompanied in all cases by ST segment deviation and dysrhythmias. Thallium-201 scintigraphy demonstrated hypoperfusion of the left ventricular free wall and septum supplied by the spastic coronary artery. The fMLP-induced epicardial vasoconstriction, ischemic electrocardiogram (ECG) changes, and thallium perfusion defects were reversed by intravenous nitroglycerin. Neither the right coronary artery nor its distribution were influenced by left coronary injection of fMLP. Additional experiments in isolated, salt solution-perfused rabbit hearts demonstrated that fMLP failed to exert direct coronary vasoconstrictor effects. These observations indicate that the nonselective inflammatory cell stimulant, fMLP, provokes arteriographically demonstrable coronary spasm with attendant myocardial hypoperfusion and ischemic ECG changes in anesthetized rabbits. Such a model may be useful in exploring the dynamic role of inflammatory cells in development of coronary spasm.

• OSTI ID: 6808793
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
DIAGNOSTIC USES
CORONARIES
BIOMEDICAL RADIOGRAPHY
SCINTISCANNING
VASOCONSTRICTION
MYOCARDIUM
PATHOLOGICAL CHANGES
ELECTROCARDIOGRAMS
INFLAMMATION
ISCHEMIA
LEUCINE
METHIONINE
NITROGLYCERIN
PHENYLALANINE
RABBITS
THALLIUM 201
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHEMICAL EXPLOSIVES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIAGRAMS
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EXPLOSIVES
HEART
HEAVY NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
MEDICINE
MUSCLES
NITRIC ACID ESTERS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
SECONDS LIVING RADIOISOTOPES
SYMPTOMS
THALLIUM ISOTOPES
USES
VASCULAR DISEASES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques