Metabolites of three structural isomers of butylbenzene in the bile of rainbow trout

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Bulletin of Environmental Contamination and Toxicology; (USA)

DOI:https://doi.org/10.1007/BF01701234· OSTI ID:6808495

Hellou, J; Ryan, A; Hodder, H J ^[1]

---

1. Dept. of Fisheries and Oceans, St. John's, Newfoundland (Canada)

In an ongoing study of the fate of petroleum hydrocarbons in fish, the authors have examined the petroleum derived metabolites which concentrate in the gall bladder bile of cunners, and rainbow trout. The metabolites obtained from the bile of rainbow trout exposed to a petroleum oil have been analyzed by GLC-MS, and several two- and three-ring aromatic alcohols were identified after hydrolysis with {beta}-glucuronidase. Another approach toward the elucidation of the structure of metabolites derived from No. 2 fuel oil relied on chemical synthesis. In another series of experiments, they investigated the type of metabolites obtained in the bile of trout exposed to three n-alkylbenzenes. Exposure of fish to a mixture containing equal amounts of these three n-alkylbenzenes showed their presence in the bile, in a ratio of 1:3:4, for n-butylbenzene, n-hexylbenzene and n-octylbenzene metabolites, respectively. The present exposure was undertaken to determine if, given aromatic hydrocarbons of same molecular weight and ring structure, a relationship could be found between the type of alkyl side chain and metabolites formed; and the influence of the amount of hydrocarbon administered during an exposure on the type of glucuronides formed. Three structural isomers of butylbenzene were chosen for the present experiment: n-butylbenzene, sec-butylbenzene and tert-butylbenzene.

• OSTI ID: 6808495
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (USA), Journal Name: Bulletin of Environmental Contamination and Toxicology; (USA) Vol. 44:3; ISSN 0007-4861; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZENE
BILE
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
ENERGY SOURCES
FISHES
FOSSIL FUELS
FUELS
GAS CHROMATOGRAPHY
HYDROCARBONS
ISOMERS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
PETROLEUM
SEPARATION PROCESSES
TROUT
UPTAKE
VERTEBRATES