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Detection of DNA single-strand breaks induced by procarcinogens in Chinese hamster ovary cells cocultured with rat hepatocytes

Journal Article · · J. Toxicol. Environ. Health; (United States)
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DNA single-strand breaks induced by procarcinogens were detected in Chinese hamster overy (CHO) cell cocultured with adult rat hepatocytes. Freshly isolated adult rat hepatocytes were added to the CHO cell culture prelabeled with (/sup 3/H) thymidine. After allowing the hepatocytes to attach on or near the CHO cells, aflatoxin B/sub 1/ or benzo(a)pyrene was added to the culture and incubated for the desired time. DNA single-strand breaks in CHO cells were measured by the alkaline elution technique. Aflatoxin B/sub 1/ induced some DNA single-strand breaks in CHO cells cultured alone, but in coculture system with hepatocytes the number of DNA single-strand breaks increased greatly. The magnitude of the increase was related to the dose and the time of exposure to aflatoxin B/sub 1/. Addition of proteinase-K to the cell lysates increased the elution of DNA compared to that of samples without proteinase-K. Benzo(a)pyrene did not induce any DNA single-strand breaks in CHO cells in the absence of liver cells, but a significant number of single-strand breaks were detected in the coculture system.

Research Organization:
Korea Advanced Inst. of Science and Tech., Seoul
OSTI ID:
6806749
Journal Information:
J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 13:1; ISSN JTEHD
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL CELLS
ANIMALS
ANTIGENS
AROMATICS
AZINES
BENZOPYRENE
BIOLOGICAL EFFECTS
CELL CULTURES
CHO CELLS
CONDENSED AROMATICS
DNA
DOSE-RESPONSE RELATIONSHIPS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
MATERIALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SOMATIC CELLS
STRAND BREAKS
THYMIDINE
TOXIC MATERIALS
TOXINS
TRITIUM COMPOUNDS
VERTEBRATES