Fluorescence quenching methods for determining equilibrium constants for polycyclic aromatic hydrocarbons binding to dissolved humic materials
A fluorescence quenching method has been developed for determining equilibrium constants for the association of pyrene, phenanthrene, and anthracene with dissolved humic and fulvic acids. The technique is based upon the observation that polycyclic aromatic hydrocarbon (PAH) fluorescence in aqueous solution is quenched upon association with humic material. Association constants are derived from the fractional decrease in fluorescence intensity as a function of added humic material with Stern-Volmer plots. This allows association constants to be measured without a separation. In addition, the technique involves a ratio measurement so the exact concentration of pollutant need not be determined. Stern-Volmer plots were linear for the three PAHs studied. Anthracene-humic association constants determined by the fluorescence quenching technique correlated reasonably well with values determined by a reverse-phase method. However, unlike the reverse-phase method the measured association constants do not depend on the concentration of dissolved organic matter.
- Research Organization:
- Univ. of New Hampshire, Durham
- OSTI ID:
- 6804204
- Journal Information:
- Environ. Sci. Technol.; (United States), Journal Name: Environ. Sci. Technol.; (United States) Vol. 20:11; ISSN ESTHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102 -- Chemical & Spectral Procedures
520200* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
AFFINITY
AQUEOUS SOLUTIONS
AROMATICS
CHEMICAL REACTION KINETICS
DATA
DISPERSIONS
EMISSION SPECTROSCOPY
EQUILIBRIUM
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
FULVIC ACIDS
HUMIC ACIDS
HYDROCARBONS
INFORMATION
KINETICS
MIXTURES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
REACTION KINETICS
SOLUTES
SOLUTIONS
SPECTROSCOPY