Application of a noval heat flux calorimeter for studying growth of Escherichia coli W in aerobic batch culture
The modification and principle of a novel heat flux calorimeter for the in situ, on-line measurement of the heat generated during microbial growth is described. Data concerning the physical characterization of the calorimeter as a fermentor, including stability and sensitivity of the heat signal, are presented. The calorimeter has been successfully applied to the study of the aerobic batch culture of Escherichia coli W on glucose under carbon and nitrogen limitation. A direct correlation between growth and heat evolution was obtained. Quantitative analysis of the data suggests that the new calorimetric technique could be used for monitoring growth and specific metabolic events, for convenient medium optimization, and as a basis for a novel fermentation process control system. 58 references.
- Research Organization:
- The Univ of Sheffield, England; Swiss Federal Institute of Technology, Lausanne, Switzerland
- OSTI ID:
- 6803866
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 28:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
BIOREACTORS
ON-LINE MEASUREMENT SYSTEMS
PROCESS CONTROL
CALORIMETERS
DESIGN
ESCHERICHIA COLI
BATCH CULTURE
TEMPERATURE MEASUREMENT
AEROBIC CONDITIONS
APPROPRIATE TECHNOLOGY
CARBON
CULTURE MEDIA
DATA
GLUCOSE
GROWTH
HEAT
HEAT TRANSFER
METABOLISM
MODIFICATIONS
MONITORING
NITROGEN
OPTIMIZATION
PRODUCTION
SENSITIVITY ANALYSIS
STABILITY
USES
ALDEHYDES
BACTERIA
CARBOHYDRATES
CONTROL
ELEMENTS
ENERGY
ENERGY TRANSFER
HEXOSES
INFORMATION
MEASURING INSTRUMENTS
MICROORGANISMS
MONOSACCHARIDES
NONMETALS
ON-LINE SYSTEMS
ORGANIC COMPOUNDS
SACCHARIDES
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550700 - Microbiology
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