Apocrine secretion by choroid plexus: isolated apical fragments synthesize protein in vitro
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6802440
Apocrine secretion was studied in the isolated rat choroid plexus. Choroid plexus explants were cultured for 1 and 16 hrs in minimal media containing S labeled methionine. Afterwards, the tissue was removed and the soluble and insoluble fractions of the incubation medium, as well as the tissue, were analyzed by transmission electron microscopy and SDS-PAGE followed by fluorography. When the explants were incubated for at least 1 hr, the nonsoluble fraction of the media contained cellular fragments morphologically similar to the apical region of the choroid plexus epithelium. An enriched fraction of these apical fragments (aposomes) was obtained and labeled with S methionine in minimal media for various periods of time. It was found that aposomes incorporated label linearly over a 22 hr period. In addition, the SDS-PAGE protein profile derived from this aposomal fraction was like the protein profile of CSF. The ratio of counts per ..mu..g DNA in the isolated aposomes indicated cells left in the aposomal fraction after removal of the choroid plexus contributed little to the incorporation of S methionine. The observation that aposomes are capable of protein synthesis suggests that aposomes may provide an additional route of entry for proteins into CSF.
- Research Organization:
- Univ. of Kansas School of Medicine, Kansas City
- OSTI ID:
- 6802440
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DRUGS
ELECTRON MICROSCOPY
ELECTROPHORESIS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MICROSCOPY
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RATS
RODENTS
SECRETION
SPINAL CORD
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DRUGS
ELECTRON MICROSCOPY
ELECTROPHORESIS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MICROSCOPY
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RATS
RODENTS
SECRETION
SPINAL CORD
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
VERTEBRATES