The GLI-Kruppel family of human genes

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.8.3104· OSTI ID:6801821

Ruppert, J M; Kinzler, K W; Wong, A J; Bigner, S H; Kao, F T; Law, M L; Seuanez, H N; O'Brien, S J; Vogelstein, B

Previous characterization of GLI, a gene found to be amplified and expressed in a subset of human brain tumors, revealed the presence of five tandem fingers related to those of Kruppel (Kr), a Drosophila segmentation gene of the gap class. The authors have used the GLI cDNA as a molecular probe to isolate related sequences from the human genome. Partial characterization of six related loci, including sequence determination, expression studies, and chromosome localization, revealed that each locus could encode a separate finger protein. The predicted proteins all had similar H-C links, i.e., a conserved stretch of 9 amino acids connecting the C-terminal histidine of one finger to the N-terminal cysteine of the next. On the basis of amino acid sequence and intron-exon organization, the genes could be placed into one of two subgroups: the GLI subgroup (with the consensus finger amino acid sequence (Y/F)XCX/sub 3/GCX/sub 3/(F/Y)X/sub 5/LX/sub 2/HX/sub 3-4/H(T/X)GEKP) or the Kr subgroup (with the consensus finger amino acid sequence (Y/F)XCX/sub 2/CX/sub 3/FX/sub 5/LX/sub 2/HXRXHTGEKP). Unlike GLI or Kr, most of the newly isolated genes were expressed in many adult tissues. The predicted proteins probably control the expression of other genes and, by analogy with Kr and GLI, may be important in human development, tissue-specific differentiation, or neoplasia.

• Research Organization: The Oncology Center, Johns Hopkins Univ. School of Medicine, Baltimore, MD (US)
• OSTI ID: 6801821
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:8
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
CELL DIFFERENTIATION
MOLECULAR BIOLOGY
PROTEINS
AMINO ACID SEQUENCE
BRAIN
CYSTEINE
DNA SEQUENCING
DROSOPHILA
GENE AMPLIFICATION
GENETIC MAPPING
MAN
NEOPLASMS
PHENOTYPE
RNA PROCESSING
SPECIES DIVERSITY
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ANIMALS
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CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DIPTERA
DISEASES
FLIES
FRUIT FLIES
INSECTS
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MAMMALS
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MOLECULAR STRUCTURE
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
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VERTEBRATES
550800* - Morphology
550200 - Biochemistry
550400 - Genetics