Susceptibility testing of Entamoeba histolytica
The growth of Entamoeba histolytica in microtiter plates in vitro in a variety of environments with reduced oxygen tensions is reported. With 3% O/sub 2/, 3% CO/sub 2/, and 94% N/sub 2/, the parasite growth in microtiter plates was identical to that in screw-capped culture tubes, as measured by (/sup 3/H)thymidine incorporation and by quantitative parasite counts. There were no significant differences between the drug concentrations necessary to inhibit parasite growth by 50% based on (/sup 3/H)thymidine incorporation vs those defined by quantitative parasite counts for the 15 antimicrobial agents tested (including seven drugs used for the treatment of amebiasis). This technique provides a reproducible method to quantitate the activity of potential antiamebic agents in vitro. The isotopic method should be of particular value in defining the metabolism of the parasite and effects of antimicrobial agents on it, whereas the morphologic method may be more valuable for workers with limited resources available to them.
- Research Organization:
- Washington Univ. School of Medicine, St Louis, MO
- OSTI ID:
- 6800398
- Journal Information:
- J. Infect. Dis.; (United States), Journal Name: J. Infect. Dis.; (United States) Vol. 148:6; ISSN JIDIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AGAR
AMOEBA
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BIOLOGICAL EFFECTS
CARBOHYDRATES
COLLOIDS
DISPERSIONS
DRUGS
GROWTH
HETEROCYCLIC COMPOUNDS
IN VITRO
INFECTIVITY
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PROTOZOA
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SARCODINA
SENSITIVITY
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS