Effect of irradiation and endogenous nucleases on rat liver chromatin
The assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,000 rad under conditions in which endogenous nucleases are inhibited and analysis of the irradiated chromatin by sucrose density gradient centrifugation gave no evidence for monosomes or oligosomes. When chromatin from irradiated nuclei was digested with micrococcal nuclease, the levels of monosomes and oligosomes were identical to those of micrococcal nuclease digests of unirradiated control nuclei. These results suggest that irradiation results in neither a direct fragmentation of linkers nor the sensitization of linkers for subsequent cleavage by micrococcal nuclease.
- Research Organization:
- Univ. of Stellenbosch, Tygerberg, South Africa
- OSTI ID:
- 6800306
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTOLYSIS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTIONS
CHEMISTRY
CHROMATIN
DECOMPOSITION
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
ENERGY RANGE
ENZYMES
ESTERASES
FRAGMENTATION
GLANDS
HYDROGEN COMPOUNDS
HYDROLASES
LIVER
MAMMALS
MASSLESS PARTICLES
MEV RANGE
MEV RANGE 01-10
MOCKUP
NUCLEASES
ORGANS
OXYGEN COMPOUNDS
PHANTOMS
PHOSPHODIESTERASES
PHOTONS
RADIATION EFFECTS
RATS
RODENTS
STRUCTURAL MODELS
VERTEBRATES
WATER