Fraction of cholesterol undergoing spontaneous exchange between small unilamellar phosphatidylcholine vesicles

Journal Article · Tue Oct 21 04:00:00 EDT 1986 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00369a056· OSTI ID:6799065

Bar, L K; Barenholz, Y; Thompson, T E

The kinetics of the spontaneous exchange of (/sup 3/H)cholesterol between small unilamellar vesicles of phosphatidylcholine has been reexamined. Although first-order exchange kinetics were observed (k = 0.0117 min-1), in good agreement with previous studies, about 20% of the total cholesterol was found to be nonexchangeable in the 8-h time frame of the experiments. The size of this nonexchangeable pool was found to depend on the type of phospholipid and the temperature. It seems probable that the two pools of cholesterol defined in these experiments reflect the complex phase structure of the cholesterol-phosphatidylcholine vesicles.

• Research Organization: Univ. of Virginia School of Medicine, Charlottesville
• OSTI ID: 6799065
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 21; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHOLESTEROL
ESTERS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LIPOSOMES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PHOSPHOLIPIDS
REACTION KINETICS
STEROIDS
STEROLS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRITIUM COMPOUNDS