Dynamical deductions from nuclear magnetic resonance relaxation measurements at the water-protein interface
Journal Article
·
· Biophys. J.; (United States)
Nuclear magnetic resonance (NMR) measurements provide both structural and dynamical information about the molecules in which nuclear resonances are observed. This manuscript addresses NMR relaxation of water protons in protein powder systems. Inclusion of magnetic communication between the water proton spins and protein proton spins leads to a clearer view of water molecule dynamics at the protein surface than has been previously available. We conclude that water molecule motion at the protein surface is somewhat slower than in the solute free solvent, but it is orders of magnitude faster than motions in a rigid ice lattice even in samples hydrated to levels well below what is generally thought to be the full hydration complement of the protein. The NMR relaxation data on lysozyme powders support a model that leaves adsorbed water very fluid at the protein surface with reorientational correlation times for the water shorter than nanoseconds.
- Research Organization:
- Univ. of Minnesota, Minneapolis
- OSTI ID:
- 6796382
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 32:1; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BIOPHYSICS
CARBOHYDRATES
CATALYSIS
ENZYMES
GLYCOSYL HYDROLASES
HYDROGEN COMPOUNDS
HYDROLASES
INTERFACES
KINETICS
LYSOZYME
MAGNETIC RESONANCE
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUCOPROTEINS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RELAXATION
RESONANCE
SACCHARIDES
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
WATER
WATER CHEMISTRY
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
BIOPHYSICS
CARBOHYDRATES
CATALYSIS
ENZYMES
GLYCOSYL HYDROLASES
HYDROGEN COMPOUNDS
HYDROLASES
INTERFACES
KINETICS
LYSOZYME
MAGNETIC RESONANCE
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUCOPROTEINS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RELAXATION
RESONANCE
SACCHARIDES
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
WATER
WATER CHEMISTRY