Production of a monoclonal antibody directed against an interferon-induced 56,000-dalton protein and its use in the study of this protein
- New York Blood Center, New York (USA)
Interferon (IFN) treatment of cells induces the synthesis of several new proteins. A hybridoma cell line producing monoclonal antibody to the IFN-induced 56,000-dalton protein has been developed. The IFN-induced 56,000-dalton protein is synthesized by a variety of different cells and in response to IFN-{alpha}, IFN-{beta}, and IFN-{gamma}. The induction of this protein is dependent on de novo RNA synthesis, since its induction is inhibited if actinomycin D and the IFNs are added to the cells simultaneously. ({sup 35}S)-labeling of IFN-treated cells at 4-h intervals at various times after the addition of the IFNs reveals that the synthesis of the 56,000-dalton protein in IFN-{alpha}-treated cells peaks within 12 h after the addition of the IFN and is no longer enhanced 20 h after exposure to the IFN. In contrast, IFN-{gamma}-treated cells continue to show an enhanced synthesis of this IFN-induced protein even after 20 h of exposure to the IFN. Thus, the synthesis of the IFN-induced 56,000-dalton protein is regulated differently by the different IFNs. When cells are treated with IFN-{alpha} or IFN-{gamma} in the presence of cycloheximide, and actinomycin D is added prior to the removal of the cycloheximide, the cells produce the IFN-induced 56,000-dalton protein and develop an antiviral state in response to both IFN-{alpha} and IFN-{gamma}. These results demonstrate that the synthesis of the 56,000-dalton protein is not dependent on the synthesis of an intermediary protein and that the establishment of an antiviral state occurs in the absence of multiple transcriptional events.
- OSTI ID:
- 6796152
- Journal Information:
- Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 62:6; ISSN 0022-538X; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL ACTIVATION
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FUNGICIDES
GROWTH FACTORS
HYBRIDOMAS
INTERFERON
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOKINES
MAMMALS
METHIONINE
MICE
MITOGENS
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PESTICIDES
PRECIPITATION
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES