Localization and synthesis of an insulin-binding region on human insulin receptor

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Journal of Protein Chemistry; (USA)

DOI:https://doi.org/10.1007/BF01025313· OSTI ID:6795723

Nakamura, S; Sakata, S; Atassi, M Z ^[1]

---

1. Baylor College of Medicine, Houston, TX (USA)

Seven regions of the alpha subunit of human insulin receptor (HIR) were synthesized and examined for their ability to bind radioiodinated insulin. A peptide representing one of these regions (namely, residues alpha 655-670) exhibited a specific binding activity for insulin. In quantitative radiometric titrations, the binding curves of {sup 125}I-labeled insulin to adsorbents of peptide alpha 655-670 and of purified placental membrane were similar or superimposable. The binding of radioiodinated insulin to peptide or to membrane adsorbents was completely inhibited by unlabeled insulin, and the inhibition curves indicated that the peptide and the membrane on the adsorbents had similar affinities. Synthetic peptides that were shorter (peptide alpha 661-670) or longer (peptide alpha 651-670) than the region alpha 655-670 exhibited lower insulin-binding activity. It was concluded that an insulin-binding region in the HIR alpha subunit resides within residues alpha 655-670. The results do not rule out the possibility that other regions of the alpha subunit may also participate in binding of HIR to insulin, with the region described here forming a face within a larger binding site.

• OSTI ID: 6795723
• Journal Information: Journal of Protein Chemistry; (USA), Journal Name: Journal of Protein Chemistry; (USA) Vol. 9:2; ISSN JPCHD; ISSN 0277-8033
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FETAL MEMBRANES
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
PLACENTA
PREGNANCY
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
VERTEBRATES