Turnover of lipids labeled by I-123 phenylpentadecanoic acid (IP) compared to C-14 palmitic acid (P)
IP has been proposed for evaluation of cardiac lipid metabolism. To elucidate the metabolic fate of IP in more detail, the authors compared its uptake and turnover to that of P in lipids extracted from heart, lung, liver, spleen and kidneys of fasted anaesthetized Wistar rats after simultaneous i.v. tracer injection. The animals were sacrificed at different time intervals until 30 min. p.i. The organs were removed and lipids were extracted with chloroform/methanol. Fractional radioactivity distribution in lipids was analyzed by TLC. I-123 and C-14 radioactivity was assayed in free fatty acid (FFFA)-, phospholipid (PL)-, diglyceride (DG)-, and triglyceride (TG)-fraction in a -spectrometer and 20 weeks later in a liquid scintillation counter. Uptake and turnover patterns of IP-and P-labeled lipids were nearly identical. The authors conclude that IP and P label essentially the same lipids and exhibit very similar lipid turnover characteristics, indicating the feasibility of metabolic studies by means of IP as tracer for lipid metabolism.
- Research Organization:
- Inst. of Nuclear Medicine, Essen
- OSTI ID:
- 6793604
- Report Number(s):
- CONF-840619-; TRN: 87-010797
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HEART
RADIOISOTOPE SCANNING
HEXADECANOIC ACID
METABOLISM
KIDNEYS
LIPIDS
DIAGNOSTIC USES
LABELLING
LIVER
LUNGS
MONOCARBOXYLIC ACIDS
SPLEEN
CARBON 14 COMPOUNDS
INTRAVENOUS INJECTION
IODINE 123
PHOSPHOLIPIDS
RATS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRIGLYCERIDES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GLANDS
HOURS LIVING RADIOISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550501 - Metabolism- Tracer Techniques