Mobilization of plasmid pHSV106 from Escherichia coli HB101 a laboratory-scale waste treatment facility

Journal Article · Tue Mar 31 23:00:00 EST 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6793488

Mancini, P; Fertels, S; Nave, D; Gealt, M A

The mobilization of plasmid pHSV106 from Escherichia coli HB101, in a laboratory model waste treatment facility, by both laboratory and indigenous wastewater strains of E. coli was monitored by transfer of antibiotic resistance characteristics and by detection of pHSV106 DNA sequences in recipient cells. The mobilization was demonstrate to occur under several different treatment conditions, such as different media composition, microbial concentrations, and waste flow rates. The herpes simplex virus thymidine kinase gene was used as a hybridization marker to confirm the occurrence of the transfer. The use of the HB101 (recA mutant) host cell implies that recA functions are unnecessary for the gene transfer.

• Research Organization: Drexel Univ., Philadelphia, PA
• OSTI ID: 6793488
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BENCH-SCALE EXPERIMENTS
CELL CONSTITUENTS
DISEASE VECTORS
DNA SEQUENCING
ESCHERICHIA COLI
GENES
HYDROGEN COMPOUNDS
INDUSTRIAL PLANTS
LIQUID WASTES
MICROORGANISMS
MOBILITY
OXYGEN COMPOUNDS
PLASMIDS
STRUCTURAL CHEMICAL ANALYSIS
WASTE PROCESSING PLANTS
WASTE WATER
WASTES
WATER