Genes expressed in the male gametophyte of flowering plants and their isolation

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.83.2.442· OSTI ID:6793463

Stinson, J R; Eisenberg, A J; Willing, R P; Pe, M E; Hanson, D D; Mascarenhas, J P

Recombinant cDNA libraries to poly(A)RNA isolated from mature pollen of Zea mays and Tradescantia paludosa have been constructed. Northern blot analyses indicate that several of the clones are unique to pollen and are not expressed in vegetative tissues. The majority, however, are expressed both in pollen and vegetative tissues. Southern hybridizations show that the pollen specific sequences in corn are present in one or a very few copies in the genome. By using several of the clones as probes, it was found that there are at least two different groups of mRNAs with respect to their synthesis. The mRNAs of the first group represented by the pollen specific clones are synthesized after microspore mitosis and increase in concentration up to maturity. The second group, exemplified by actin mRNA, begins to accumulate soon after meiosis, reaches its maximum by later pollen interphase, and decreases thereafter. Although the actin mRNA and the pollen specific mRNAs studied show very different patterns of initiation of synthesis and accumulation during pollen development, the rates of decline in these mRNAs during the first 60 minutes of germination and pollen tube growth in Tradescantia are similar and reflect the previously observed declines in rates of protein synthesis during this period.

• Research Organization: State Univ. of New York, Albany
• OSTI ID: 6793463
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 83:2; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CELL DIVISION
CEREALS
DNA
DNA SEQUENCING
GAMETES
GENE RECOMBINATION
GENES
GERM CELLS
GRASS
HYBRIDIZATION
MAIZE
MESSENGER-RNA
MITOSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANT BREEDING
PLANTS
POLLEN
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRADESCANTIA
TRANSCRIPTION