Characterization and regulation of an extrahepatic form of rabbit FAD-containing monooxygenase (FMO)
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6792349
A form of FMO has been purified from lung microsomes of pregnant rabbit and demonstrated to be catalytically and immunochemically distinct from the liver enzyme. The amount and activity of rabbit lung FMO increase 5-fold during late gestation. Immunoquantitation by Western blotting has demonstrated that rabbit lung FMO is also expressed in kidney and bladder but, as previously observed, is not detectable in liver. Goat anti-rabbit lung FMO-IgG specifically immunoprecipitates a protein, with an identical molecular weight on SDS-PAGE, from in vitro rabbit reticulocyte lysate translation systems (/sup 35/S-met) charged with RNA extracted from lungs of pregnant rabbits. The addition of unlabelled rabbit lung FMO produced a concentration-dependent competition of the immunoprecipitated /sup 35/S-labelled protein. These results demonstrate that this rabbit extrahepatic FMO is translated as the mature protein. Further studies are needed to examine the regulation of this rabbit FMO in fetal lung and maternal lung, kidney and bladder at the genetic level.
- Research Organization:
- Medical College of Wisconsin, Milwaukee
- OSTI ID:
- 6792349
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLADDER
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUNGS
MAMMALS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PREGNANCY
PURIFICATION
RABBITS
RADIOISOTOPES
RESPIRATORY SYSTEM
SULFUR 35
SULFUR ISOTOPES
URINARY TRACT
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLADDER
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUNGS
MAMMALS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PREGNANCY
PURIFICATION
RABBITS
RADIOISOTOPES
RESPIRATORY SYSTEM
SULFUR 35
SULFUR ISOTOPES
URINARY TRACT
VERTEBRATES