Membrane H/sup +/ conductance of Clostridium thermoaceticum and Clostridium acetobutylicum: evidence for electrogenic Na/sup +/H/sup +/ antiport in Clostridium thermoaceticum

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6791479

Terracciano, J S; Schreurs, W J.A.; Kashket, E R

H/sup +/ conductance in de-energized cells of Clostridium thermoaceticum and Clostridium acetobutylicum was determined from the rate of realkalinization of the medium after an acid pulse. In both organisms, cell membrane proton permeability was increased by fermentation end products and ionophores. In C. thermoaceticum, H/sup +/ conductance was increased by Na/sup +/ ions compared with K/sup +/ as counterions. In these cells, addition of Na/sup +/, but not K/sup +/, elicited efflux of H/sup +/;H/sup +/ efflux was stimulated by SCN/sup -/ and decreased by various ionophores. The authors concluded that C. thermoaceticum possesses an electrogenic Na/sup +//H/sup +/ antiporter. In contrast, C. acetobutylicum cells did not have an electrogenic Na/sup +//H/sup +/ antiporter.

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 6791479
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
BACTERIA
BARYONS
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
CELL MEMBRANES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
ELEMENTARY PARTICLES
FERMIONS
GROWTH
HADRONS
MEMBRANE TRANSPORT
MEMBRANES
METHANOGENIC BACTERIA
MICROORGANISMS
NUCLEONS
PERMEABILITY
POTASSIUM COMPOUNDS
PROTONS
RESPONSE MODIFYING FACTORS
SODIUM COMPOUNDS