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Title: Selective incorporation of various C-22 polyunsaturated fatty acids in Ehrlich ascites tumor cells

Abstract

Three /sup 14/C-labeled 22-carbon polyunsaturated fatty acids, 7,10,13,16-(/sup 14/C)docosatetraenoic acid (22:4(n-6)), 7,10,13,16,19-(/sup 14/C)docosapentaenoic acid (22:5(n-3)), and 4,7,10,13,16,19-(/sup 14/C)docosahexaenoic acid (22:6(n-3)), were compared with (/sup 3/H)arachidonic acid (20:4(n-6) and (14C)linoleic acid (18:2(n-6)) to characterize their incorporation into the lipids of Ehrlich ascites cells. The relatively rapid incorporation of the labeled 22-carbon acids into phosphatidic acid indicated that substantial amounts of these acids may be incorporated through the de novo pathway of phospholipid synthesis. In marked contrast to 20:4(n-6), the 22-carbon acids were incorporated much less into choline glycerophospholipids (CGP) and inositol glycerophospholipids (IGP). No selective preference was apparent for the (n-3) or (n-6) type of fatty acids. The amounts of the acids incorporated into diacylglycerophosphoethanolamine were in the order of: 22:6(n-3) greater than 20:4(n-6) much greater than 22:5(n-3) greater than or equal to 22:4(n-6) greater than 18:2(n-6), whereas for alkylacylglycerophosphoethanolamine they were in the order of: 22:4(n-6) greater than 22:6(n-3) greater than 22:5(n-3) much greater than 20:4(n-6) greater than 18:2(n-6). Of the mechanisms possibly responsible for the selective entry of 22-carbon acids into ethanolamine glycerophospholipids, the most reasonable explanation was that the cytidine-mediated ethanolamine phosphotransferase may have a unique double selectivity: for hexaenoic species of diacylglycerol and for 22-carbon polyunsaturated fattymore » acid-containing species of alkylacylglycerol. The relative distribution of fatty acids between newly incorporated and already maintained lipid classes suggested that IGP may function in Ehrlich cells as an intermediate pool for the retention of polyunsaturated fatty acids in glycerolipids.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Teikyo Univ., Kanagawa, Japan
OSTI Identifier:
6791359
Alternate Identifier(s):
OSTI ID: 6791359
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Lipid Res.; (United States); Journal Volume: 11
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBOXYLIC ACIDS; STRUCTURE-ACTIVITY RELATIONSHIPS; TUMOR CELLS; METABOLISM; CARBON 14 COMPOUNDS; EHRLICH ASCITES TUMOR; MICE; PHOSPHOLIPIDS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; ESTERS; EXPERIMENTAL NEOPLASMS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; RODENTS; VERTEBRATES 550501* -- Metabolism-- Tracer Techniques

Citation Formats

Masuzawa, Y., Okano, S., Waku, K., Sprecher, H., and Lands, W.E. Selective incorporation of various C-22 polyunsaturated fatty acids in Ehrlich ascites tumor cells. United States: N. p., 1986. Web.
Masuzawa, Y., Okano, S., Waku, K., Sprecher, H., & Lands, W.E. Selective incorporation of various C-22 polyunsaturated fatty acids in Ehrlich ascites tumor cells. United States.
Masuzawa, Y., Okano, S., Waku, K., Sprecher, H., and Lands, W.E. Sat . "Selective incorporation of various C-22 polyunsaturated fatty acids in Ehrlich ascites tumor cells". United States. doi:.
@article{osti_6791359,
title = {Selective incorporation of various C-22 polyunsaturated fatty acids in Ehrlich ascites tumor cells},
author = {Masuzawa, Y. and Okano, S. and Waku, K. and Sprecher, H. and Lands, W.E.},
abstractNote = {Three /sup 14/C-labeled 22-carbon polyunsaturated fatty acids, 7,10,13,16-(/sup 14/C)docosatetraenoic acid (22:4(n-6)), 7,10,13,16,19-(/sup 14/C)docosapentaenoic acid (22:5(n-3)), and 4,7,10,13,16,19-(/sup 14/C)docosahexaenoic acid (22:6(n-3)), were compared with (/sup 3/H)arachidonic acid (20:4(n-6) and (14C)linoleic acid (18:2(n-6)) to characterize their incorporation into the lipids of Ehrlich ascites cells. The relatively rapid incorporation of the labeled 22-carbon acids into phosphatidic acid indicated that substantial amounts of these acids may be incorporated through the de novo pathway of phospholipid synthesis. In marked contrast to 20:4(n-6), the 22-carbon acids were incorporated much less into choline glycerophospholipids (CGP) and inositol glycerophospholipids (IGP). No selective preference was apparent for the (n-3) or (n-6) type of fatty acids. The amounts of the acids incorporated into diacylglycerophosphoethanolamine were in the order of: 22:6(n-3) greater than 20:4(n-6) much greater than 22:5(n-3) greater than or equal to 22:4(n-6) greater than 18:2(n-6), whereas for alkylacylglycerophosphoethanolamine they were in the order of: 22:4(n-6) greater than 22:6(n-3) greater than 22:5(n-3) much greater than 20:4(n-6) greater than 18:2(n-6). Of the mechanisms possibly responsible for the selective entry of 22-carbon acids into ethanolamine glycerophospholipids, the most reasonable explanation was that the cytidine-mediated ethanolamine phosphotransferase may have a unique double selectivity: for hexaenoic species of diacylglycerol and for 22-carbon polyunsaturated fatty acid-containing species of alkylacylglycerol. The relative distribution of fatty acids between newly incorporated and already maintained lipid classes suggested that IGP may function in Ehrlich cells as an intermediate pool for the retention of polyunsaturated fatty acids in glycerolipids.},
doi = {},
journal = {J. Lipid Res.; (United States)},
number = ,
volume = 11,
place = {United States},
year = {Sat Nov 01 00:00:00 EST 1986},
month = {Sat Nov 01 00:00:00 EST 1986}
}