Dynamics of fd coat protein in the bacteriophage

Journal Article · Mon Feb 09 23:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00377a028· OSTI ID:6790824

Colnago, L A; Valentine, K G; Opella, S J

The dynamics of the coat protein in fd bacteriophage are described with solid-state /sup 15/N and /sup 2/H NMR experiments. The virus particles and the coat protein subunits are immobile on the time scales of the /sup 15/N chemical shift anisotropy (10/sup 3/ Hz) and /sup 2/H quadrupole (10/sup 6/ Hz) interactions. Previously the authors have shown that the Trp-26 side chains is immobile, that the two Tyr and three Phe side chains undergo only rapid two fold jump motions about their C/sub ..beta../-C/sub ..gamma../ bond axis and that most of the backbone peptide linkages are highly constrained but do undergo rapid small amplitude motions in the coat protein subunits in the virus particles. In this paper, the authors demonstrate that the four N-terminal residues of the coat protein subunits are highly mobile, since both backbone and side-chain sites of these residues undergo large amplitude motions that are rapid on the time scales of the solid-state NMR experiments. In addition, the dynamics of the methyl-containing aliphatic residues Ala, Leu, Thr, and Met are analyzed. Large amplitude jump motions are observed in nearly all of these side chains even through, with the exception of the N-terminal residue Ala-1, their backbone peptide linkages are highly constrained. The established information about the dynamics of the structural form of fd coat protein in the virus particle is summarized qualitatively; backbone sites are found to be either immobile or highly mobile, while side-chain sites are found either to be immobile or to undergo apparently well-defined high-frequency jump motions.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 6790824
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:3; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALANINES
AMINO ACIDS
ANISOTROPY
BACTERIOPHAGES
CARBOXYLIC ACIDS
DEUTERIUM COMPOUNDS
DRUGS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
METHIONINE
MICROORGANISMS
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTEINS
RESONANCE
SPECTRA
STABLE ISOTOPES
THREONINE
VALINE
VIRUSES