Isolation and characterization of mink lung epithelial cell mutants resistant to transforming growth factor. beta
Mink lung epithelial cells resistant to growth inhibition by transforming growth factor ..beta.. (TGF-..beta..) have been isolated by chemical mutagenesis and growth in the presence of platelet extracts enriched in TGF-..beta... Several resistant clones were isolated, at least one of which stably retained its resistance to TGF-..beta.. when grown in the absence of the factor. The cells of this clone were similar to the parent cells in morphology and growth properties. However, unlike the parent cells, the resistant cells did not show any of the following responses to /sup 125/I TGF-..beta..: (1) inhibition of DNA synthesis and proliferation; (2) morphological changes involving increased cell spreading; or (3) stimulation of synthesis of a 48-kilodalton secreted /sup 35/S-protein. The resistant cells do, however, retain a functional TGF-..beta.. receptor. The TGF-..beta.. resistant cell lines may be useful in genetic studies designed to identify the biochemical events required for inhibition of epithelial cell growth by this factor.
- Research Organization:
- Vanderbilt Univ. School of Medicine, Nashville, TN
- OSTI ID:
- 6790470
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 130:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
EPITHELIUM
MORPHOLOGICAL CHANGES
MUTANTS
BIOCHEMISTRY
PROTEINS
RADIORECEPTOR ASSAY
BLOOD PLATELETS
CELL PROLIFERATION
DNA REPLICATION
GROWTH
IODINE 125
LUNGS
METHIONINE
SULFUR 35
THYMIDINE
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL TISSUES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RIBOSIDES
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550301 - Cytology- Tracer Techniques