In vitro dissolution of curium oxide using a phagolysosomal simulant solvent system
- Inhalation Toxicology Research Institute, Albuquerque, NM (United States)
- Texas A M Univ., College Station, TX (United States)
Detailed study of actinide oxide behavior in alveolar macrophages (AM) in vitro is limited because of the short life span of these cells in culture. We created an in vitro dissolution system that could mimic the acidic phagolysosomal environment for the actinide and be maintained for an indefinite period so that dissolution of more insoluble materials could be measured. The dissolution system for this investigation, consisting of nine different solutions of HCl and the chelating agent diethylenetriamine pentaacetate (DTPA) in distilled water, is called the phagolysosomal simulant solvent (PSS). In this system, both the pH and the amount of DTPA were varied. We could observe the effect of altering pH within a range of 4.0-6.0 (similar to that of the phagolysosome) and the effect of the molar ratio of DTPA to curium at 1000: 1, 100;1, or 10:1. We chose curium sesquioxide ([sup 244]Cm[sub 2]O[sub 3]) to validate the PSS for actinide dissolution versus that occurring in AM in vitro because it dissolves significantly in less than 1 week. The polydisperse [sup 244]Cm[sub 2]O[sub 3] aerosol was generated, collected on filters, resuspended, and added to the PSS solutions and to cultured canine AM. By comparing dissolution in the two systems directly, we hoped to arrive at an optimum PSS for future dissolution studies. PSS and cell culture samples were taken daily for 7 days after exposure and tested for the solubilized curium. The amount of soluble material was determined by ultracentrifugation to separate the insoluble CM[sub 2]O[sub 3] from the soluble curium in the PSS solutions and filtration for the cell-containing material. After separating the soluble and insoluble fractions, the samples were analyzed using alpha liquid scintillation counting. Time-dependent dissolution measurements from the PSS/AM showed that the CM[sub 2]O[sub 3] dissolution was similar for both the PSS solutions and the cultured AM. 13 refs., 4 tabs.
- DOE Contract Number:
- AC04-76EV01013
- OSTI ID:
- 6790322
- Journal Information:
- Environmental Health Perspectives; (United States), Vol. 97; ISSN 0091-6765
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACTINIDES
DISSOLUTION
AQUEOUS SOLUTIONS
FEASIBILITY STUDIES
MACROPHAGES
METABOLIC ACTIVATION
CURIUM OXIDES
DTPA
PH VALUE
SCINTILLATION COUNTING
ACTINIDE COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
CARBOXYLIC ACIDS
CHALCOGENIDES
CHELATING AGENTS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
CURIUM COMPOUNDS
DISPERSIONS
DRUGS
ELEMENTS
METALS
MIXTURES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
RADIOPROTECTIVE SUBSTANCES
SOLUTIONS
SOMATIC CELLS
TRANSPLUTONIUM COMPOUNDS
TRANSURANIUM COMPOUNDS
560150* - Radiation Effects on Animals
550200 - Biochemistry