Increased synthetic capacity for metallothionein in cultured liver cells following dimethyl sulfoxide pretreatment
Journal Article
·
· Exp. Cell Res.; (United States)
Cultured TRL 1215 cells in log phase of growth were exposed to dimethyl sulfoxide (DMSO; 14-280 mM) followed 48 hr later by cadmium (10 ..mu..m). Intracellular concentrations of metallothionein (MT) were measured 24 hr after cadmium addition. Cadmium alone caused a 10-fold increase in the levels of MT, while DMSO alone had no effect on cellular MT levels. DMSO pretreatment followed by cadmium exposure, however, resulted in MT levels that were elevated by a factor of as much as 25-fold those observed in control cells. Concurrent treatment with the DNA synthesis inhibitor hydroxyurea (HU) eliminated the enhancing effect of DMSO pretreatment on cadmium induction of MT, indicating the requirement of DNA synthesis. An enhancement of the cellular accumulation of the metal ion did not account for the increased cadmium-induced MT synthesis in DMSO-pretreated cells as these cells did not show significantly increased uptake of cadmium during the initial period of exposure. DMSO pretreatment enhances cadmium induction of MT synthesis through a mechanism that appears to be dependent on the synthesis of DNA.
- Research Organization:
- National Cancer Institute, Frederick, MD
- OSTI ID:
- 6785896
- Journal Information:
- Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 169:1; ISSN ECREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CELL CULTURES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
DMSO
DNA REPLICATION
HALIDES
HALOGEN COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
LIVER CELLS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RATS
RODENTS
SOMATIC CELLS
SULFOXIDES
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CELL CULTURES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
DMSO
DNA REPLICATION
HALIDES
HALOGEN COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
LIVER CELLS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RATS
RODENTS
SOMATIC CELLS
SULFOXIDES
VERTEBRATES