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Excision repair of 5,6-dihydroxydihydrothymine from the DNA of Micrococcus radiodurans

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575286· OSTI ID:6784516
;

One of the major ionizing radiation products, 5,6-dihydroxydihydrothymine (thymine glycol), was measured in the DNA of Micrococcus radiodurans following exposure of cells to 6.8-MeV electrons or 254-nm ultraviolet light. Removal of 5,6-dihydroxydihydrothymine was measured in both an ionizing radiation-sensitive strain (262) and a highly radioresistant strain (the wild type W/sup +/) of Micrococcus radiodurans. Within 30 min of incubation (33/sup 0/C) following exposure to ultraviolet light (2400 J/m/sup 2/) approximately 60% of the thymine glycols were excised, whereas in the case of ionizing radiation (250 krad) only 35% were removed from the cellular DNA of the wild-type strain. In contrast less than 50% of the thymine glycols were excised from the sensitive strain. The amount of DNA degradation induced by radiation was less than 10% in both strains. The results suggest a possible correlation between reduced excision repair of base damage and increased radiation sensitivity.

Research Organization:
Roswell Park Memorial Inst., Buffalo, NY
OSTI ID:
6784516
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 83:2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550701 -- Microbiology-- Tracer Techniques
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AZINES
BACTERIA
BEAMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DATA
DNA
ELECTROMAGNETIC RADIATION
ELECTRON BEAMS
EXPERIMENTAL DATA
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
IONIZING RADIATIONS
LABELLED COMPOUNDS
LEPTON BEAMS
MICROCOCCUS
MICROCOCCUS RADIODURANS
MICROORGANISMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLE BEAMS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
THYMINE
TIME DEPENDENCE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
URACILS