Heparin interferes with the radioenzymatic and homogeneous enzyme immunoassays for aminoglycosides

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6783301

Krogstad, D J ^[1]; Granich, G G; Murray, P R; Pfaller, M A; Valdes, R

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1. Barnes Hospital, St. Louis, MO

Heparin interferes with measurement of aminoglycosides in serum by biological, radioenzymatic, and homogeneous enzyme immunoassay techniques, but not with radioimmunoassay. At concentrations greater than or equal to 10/sup 5/ and greater than or equal to 3 X 10/sup 6/ USP units/L, respectively, it interferes with the radioenzymatic assay by inhibiting the gentamicin 3-acetyltransferase and kanamycin 6'-acetyltransferase enzymes used in the assay. It interferes with the homogeneous enzyme immunoassays for gentamicin and tobramycin (at concentrations greater than or equal to 10/sup 5/ and greater than or equal to10/sup 4/ USP units/L, respectively), but not with the commercially available homogeneous enzyme immunoassays for other drugs. Heparin interference with the homogeneous enzyme immunoassay for aminoglycosides requires both the heparin polyanion and glucose-6-phosphate dehydrogenase bound to a cationic aminoglycoside. This interference can be reproduced with dextran sulfate (but not dextran), and does not occur with free enzyme (glucose-6-phosphate dehydrogenase) alone. Heparin interference with these two assays and at concentrations that may be present in intravenous infusions or in seriously underfilled blood-collection tubes is described. (JMT)

• OSTI ID: 6783301
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
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ANTICOAGULANTS
CARBOHYDRATES
CHEMICAL ANALYSIS
DRUGS
HEMATOLOGIC AGENTS
HEPARIN
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TRACER TECHNIQUES