Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella montevideo

Journal Article · · J. Immunol.; (United States)
OSTI ID:6778305
; ; ;
The population of LPS molecules on Salmonella montevideo that bind C3 during alternative pathway activation in serum was studied. LPS molecules of Salmonella are composed of lipid A:core oligosaccharide (one copy per molecule), substituted by an O-polysaccharide (O-PS) side chain, which is a linear polymer of 0 to >60 O-antigen repeat units containing mannose. A mutant of S. montevideo called SL5222 that inserts galactose only into core oligosaccharide and mannose only into O-antigen sub-units was grown with (/sup 3/H)mannose and (/sup 14/C)galactose, so that LPS molecules bearing large numbers of O-antigen subunits have high /sup 3/H to /sup 14/C ratios, whereas molecules with few O-antigen subunits have lower /sup 3/H to /sup 14/C ratios. The authors postulate that preferential attachment of C3 to long-chain LPS in SL5222 results because long-chain LPS molecules sterically hinder shorter chain LPS molecules from macromolecules. This study provides direct proof that the O-PS of LPS sterically hinders access of large molecules to the outer membrane and indicates that the LPS coat of these bacteria functions as a barrier against large protein molecules.
Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
6778305
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:2; ISSN JOIMA
Country of Publication:
United States
Language:
English

Similar Records

Associations of Escherichia coli K-12 OmpF trimers with rough and smooth lipopolysaccharides
Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Journal of Bacteriology; (USA) · OSTI ID:6511251
O-linked oligosaccharides on insulin receptor
Journal Article · Thu Jan 31 23:00:00 EST 1991 · Diabetes; (USA) · OSTI ID:6062326
Clinical and veterinary isolates of Salmonella enterica serovar Enteritidis defective in lipopolysaccharide O-chain polymerization
Journal Article · Sat May 01 00:00:00 EDT 1999 · Applied and Environmental Microbiology · OSTI ID:346834

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBON 14
CARBON ISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
COMPLEMENT
DYNAMIC FUNCTION STUDIES
EVEN-EVEN NUCLEI
GALACTOSE
HEXOSES
HYDROGEN ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOPOLYSACCHARIDES
MANNOSE
MEMBRANES
MICROORGANISMS
MOLECULAR WEIGHT
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SALMONELLA
TRITIUM
YEARS LIVING RADIOISOTOPES