Saccharification of wheat-straw cellulose by enzymatic hydrolysis following fermentative and chemical pretreatment

Conference · Mon Dec 31 23:00:00 EST 1979 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6775260

Detroy, R W; Lindenfelser, L A; Julian, G Jr; Orton, W L

In our investigations, wheat straw fermentations were conducted using the edible, white-rot fungus commonly known as the oyster mushroom, Pleurotus ostreatus (Jacq. ex Fr.) Kummer, as fermentation organism. Fermented substrates were evaluated for degree of lignin and cellulose degradation and saccharification. In addition, since our primary objective in the P. ostreatus fermentation was to increase the amount of availabile cellulose in straw for further fermentation, cellulose hydrolysis rates were determined. Cellulose conversion to fermentable sugar was also determined on chemically modified straws by subjecting them to enzymatic hydrolysis. Progress and extent of delignification was follwed also by scanning electron microscopy (SEM), and structural changes were determined in treated-straw substrates.

• Research Organization: Dept. of Agriculture, Peoria, IL
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6775260
• Report Number(s): CONF-791072-
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 10; ISSN BIBSB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOCONVERSION
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
DECOMPOSITION
DELIGNIFICATION
ELECTRON MICROSCOPY
ENZYMATIC HYDROLYSIS
FERMENTATION
FUNGI
HYDROLYSIS
KINETICS
LIGNIN
LYSIS
MICROSCOPY
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
SCANNING ELECTRON MICROSCOPY
SOLVOLYSIS
STRAW