Effect of acetylation on monoclonal antibody ZCE-025 Fab': Distribution in normal and tumor-bearing mice
Journal Article
·
· Journal of Biological Response Modifiers; (USA)
OSTI ID:6773995
- Veterans Administration Medical Center, San Diego, CA (USA)
Studies were performed to determine in vitro and in vivo effects of acetylation on Fab' fragments of ZCE-025, a monoclonal anti-CEA antibody. Isoelectric focusing revealed a drop in isoelectric point of 1.7 pI units following acetylation. Biodistribution studies of acetylated and nonacetylated (111In)Fab' were performed in normal BALB/c mice and in nude mice bearing the T-380 CEA-producing human colon tumor. The acetylated fragments remained in the vascular compartment longer and had significantly diminished renal uptake of 111In compared to controls. While acetylation itself effected a 50% drop in immunoreactivity, tumor uptake of the acetylated and nonacetylated 111In-labeled Fab' fragments was comparable, with the exception of one data point, through 72 h.
- OSTI ID:
- 6773995
- Journal Information:
- Journal of Biological Response Modifiers; (USA), Journal Name: Journal of Biological Response Modifiers; (USA) Vol. 9:2; ISSN 0732-6580; ISSN JBRMD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLATION
ACYLATION
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CEA
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL NEOPLASMS
FRENCH ORGANIZATIONS
GLOBULINS
IMMUNOGLOBULINS
IN VITRO
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NATIONAL ORGANIZATIONS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
TISSUE DISTRIBUTION
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLATION
ACYLATION
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CEA
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL NEOPLASMS
FRENCH ORGANIZATIONS
GLOBULINS
IMMUNOGLOBULINS
IN VITRO
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NATIONAL ORGANIZATIONS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
TISSUE DISTRIBUTION
VERTEBRATES