Photosynthetic regeneration of ATP using a strain of thermophilic blue-green algae
Photosynthetic ATP accumulation was shown in the presence of exogenous ADP plus ortho-phosphate on illumination to the intact cells of a strain of thermophilic blue-green algae isolated from Matsue hot springs, Mastigocladus sp. Kinetic studies of various effectors on the ATP accumulation proved that the ATP synthesis depends mainly on the cyclic photophosphorylation system around photosystem I (PS-I) in the algal cells. The temperature and pH optima for the accumulation were found at 45 degrees C and pH 7.5. Maximum yield was obtained with light intensity higher than 15 mW/squared cm. Borate ion exerted pronounced enhancement on the ATP synthesis. With a continuous reactor at a flow rate of 1 ml/hour at 45 degrees C and pH 7.5, efficient photoconversion of ADP (2mM, at substrate reservoir) to ATP (1mM, at product outlet) has been maintained for a period of 2.5 days, though the efficiency has decreased in a further 2-day period to the level of 0.5 mM ATP/9.5 h of residence time. (Refs. 24).
- Research Organization:
- Lab Biochemistry, Coll Agriculture, Shimane Univ Nishikawazu-cho, Matsue, 690, Japan
- OSTI ID:
- 6771571
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 24:2
- Country of Publication:
- United States
- Language:
- English
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09 BIOMASS FUELS
14 SOLAR ENERGY
ALGAE
ATP
CONTINUOUS CULTURE
BIOSYNTHESIS
ADP
CHLOROPLASTS
KINETICS
PH VALUE
PHOSPHORUS
PHOTOSYNTHESIS
TEMPERATURE EFFECTS
THERMOPHILIC CONDITIONS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELEMENTS
NONMETALS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PLANTS
SYNTHESIS
550700* - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
140505 - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)