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Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1/sup +/2/sup -//9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form eosinophil colonies. We have isolated a cDNA sequence that expresses IgA-enhancing factor and eosinophil colony-stimulating factor activities from a cDNA library prepared from activated Ly1/sup +/2/sup -//9 cells. Based on homology with the mouse cDNA sequence, a human cDNA sequence coding for an interleukin with IgA-enhancing factor and eosinophil colony-stimulating factor activities was isolated from a cDNA library prepared from a human T-cell clone stimulated with anti-T3 antibody and phorbol 12-myristate 13-acetate. DNA sequence analyses revealed that mouse and human cDNA clones encode proteins of 133 and 134 amino acids, respectively, that are identical to cDNA clones encoding the T-cell replacing factor I and B-cell growth factor II activities. These results establish that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cell and eosinophils.
Research Organization:
DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA (USA)
OSTI ID:
6771406
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:21; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CARCINOGENS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DRUGS
ELECTROPHORESIS
EOSINOPHILS
ESTERS
EVEN-ODD NUCLEI
GLOBULINS
GROWTH
GROWTH FACTORS
IMMUNOGLOBULINS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
METHIONINE
MICE
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES