Diffusion-enhanced lanthanide energy transfer studies of protein prosthetic groups

Journal Article · Wed Oct 01 04:00:00 EDT 1980 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(80)84942-3· OSTI ID:6761272

Meares, C F; Yeh, S M; Rice, L S

A long-lived luminescent solute in aqueous solution (e.g., /sup 5/D/sub 4/ terbium, tau approx. =10/sup -3/s) can donate its excitation energy to a chromophore such as a protein prosthetic group by, e.g., the radiationless dipolar mechanism of Foerster. However, in contrast to the usual energy-transfer experiment, a donor with a 10/sup -3/s lifetime can diffuse extensively through the solution and, in a time scale short compared to its excited lifetime, sample all permitted locations with respect to chromophoric acceptors. As recently indicated by Thomas et al. energy transfer in this rapid-diffusion limit can permit direct measurement of the allowed distance of closest approach of small solute molecules to chromophores which may be buried within proteins or membranes.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6761272
• Journal Information: Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 32:1; ISSN BIOJA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AQUEOUS SOLUTIONS
BIOCHEMICAL REACTION KINETICS
DIFFUSION
DISPERSIONS
ELEMENTS
ENERGY TRANSFER
KINETICS
LUMINESCENCE
METALS
MIXTURES
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PROTEINS
RARE EARTHS
REACTION KINETICS
SOLUTIONS
STRUCTURAL CHEMICAL ANALYSIS