Biochemical nature of cellulases from mutants of Trichoderma reesei

Conference · Mon Dec 31 23:00:00 EST 1979 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6760851

Montenecourt, B S ^[1]; Kelleher, T J; Eveleigh, D E; Pettersson, L G

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1. State Univ. of New Jersey, New Brunswick

The cellulases of two new mutants of T. reesei, RUT-NG14 and RUT-C30, have been examined with respect to the separation and biochemical characterization of the various components in the cellulase complex. The cellulase of both mutants has been shown to contain enhanced proportions of a cellobiohydrolase by quantitative immune precipitation. Application of ion-exchange high-pressure liquid chromatography protein separations affords a rapid (30 min) and simple method of separating and comparing cellulase components from potential high-yielding cellulase mutants.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6760851
• Report Number(s): CONF-791072-
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 10; ISSN BIBSB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MUTANTS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA