Imaging of cutaneous T cell lymphoma (CTCL) with In-111-T101 monoclonal antibody

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6760562

Carrasquillo, J A; Bunn, P A; Keenan, A M; Reynolds, J C; Schroff, R W; Foon, K A; Ming-Hsu, S; Gazdar, A F; Mulshine, J M; Perentesis, P

T101 is a murine monoclonal antibody (MoAb), IgC2a, directed against a cell surface pan T-cell antigen present in high concentration in CTCL cells. In-111 labelling was performed with a modification of the Krejcarek method (Hybritech, Inc.). I mg of DTPA conjugated T101 was labeled with 5 mCi, with a mean incorporation of 95%. Immunoreactivity was preserved, mean 88%. In vivo, less than 3.6% of the injected dose was on circulating transferrin. 11 patients (pts) received 2-6h intravenous infusion of 1 mg (5 pts), 10 mg (3 pts), 50 mg (3 pts) of In-111 T101. By 24h all pts showed avid uptake in pathologically or clinically involved nodes and erythroderma including several previously unsuspected nodal regions. Skin plaques were not visualized. In addition, there was localization in liver, spleen and bone marrow. Concentration of In-111 in biopsied nodes was 0.01, 0.02 and 0.03% of the injected dose per gram. Control studies with In-111Cl/sub 3/ or a nonspecific MoAb, 9.2.27, did not concentrate in nodes or skin disease. No dose dependent differences in tumor localization was seen although blood clearance was prolonged for doses less than or equal to 10 mgs of T101. All pts receiving less than or equal to 10 mgs developed transient itching, urticaria and chills. 1 of 8 pts tested had an antimouse immune response. Modulation of the antigen from circulating T-cells, skin and nodes was seen. This study shows the feasibility of imaging CTCL pts with In-111 T101 and suggest a potential for radioimmunotherapy.

• Research Organization: Nuclear Medicine Dept., NCI-Medical Oncology Branch, Biological Response Modifier Program, NIH, Bethesda, MD
• OSTI ID: 6760562
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMAL TISSUES
ANTIBODIES
ANTIGENS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BIOMEDICAL RADIOGRAPHY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DRUGS
FUNCTIONS
GLANDS
HEMATOPOIETIC SYSTEM
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
LIVER
LYMPHOCYTES
LYMPHOMAS
MATERIALS
MEDICINE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
ORGANS
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOLOGY
RADIOPHARMACEUTICALS
RADIOTHERAPY
RETENTION FUNCTIONS
SCINTISCANNING
SKIN
SKIN DISEASES
SOMATIC CELLS
SPLEEN
THERAPY
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
UPTAKE