Single laboratory evaluation of the hydrogen oxidation soil bioassay

Technical Report · Fri Jun 01 00:00:00 EDT 1984

OSTI ID:6758945

Rogers, R D

The Hydrogen Oxidation Soil Bioassay was single laboratory tested as a potential method for monitoring hazardous wastes and hazardous waste sites. The bioassay is based on the rate of hydrogen consumption by soil microorganisms. Oxidation of hydrogen to water is inhibited when various pollutants are present in the soil and the rate of this reaction can be used as an indication of potential hazard to the soil ecosystem. The single laboratory evaluation included ruggedness testing, a determination of method sensitivity and precision, and tests to determine the limits of reliable measurement. Aqueous solutions of mercuric chloride were used as sample material during the evaluation. Some preliminary tests were also conducted using both organic compounds and actual hazardous waste samples. The bioassay was found to be 'rugged' in the sense that modest procedural variations did not produce an altered test result. The complete Hydrogen Oxidation Soil Bioassay protocol, the results of chemical analyses conducted on actual samples that were used during the single laboratory test, and the preliminary bioassay responses to different types of sample material are included in the project report.

• Research Organization: EG and G Idaho, Inc., Idaho Falls (USA)
• OSTI ID: 6758945
• Report Number(s): PB-84-211317
• Country of Publication: United States
• Language: English

Related Subjects

510200* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
BENCH-SCALE EXPERIMENTS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
ELEMENTS
EQUIPMENT
EVALUATION
HAZARDOUS MATERIALS
HYDROGEN
KINETICS
LABORATORY EQUIPMENT
MATERIALS
MICROORGANISMS
NONMETALS
OXIDATION
REACTION KINETICS
SOILS