Chromosomal localization and sequence analysis of a human episomal sequence with in vitro differentiating activity

Journal Article · Sun May 01 00:00:00 EDT 1994 · Somatic Cell and Molecular Genetics; (United States)

DOI:https://doi.org/10.1007/BF02254757· OSTI ID:6758613

Boccaccio, C; Deshatrette, J; Meunier-Rotival, M ^[1]; Apiou, F ^[2]; Alurias, A ^[3]

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1. INSERM U347, Kremlin-Bicetre (France)
2. CNRS URA 620, Paris (France)
3. Institut Curie, Paris (France)

The genomic fragment carrying the human activator of liver function, previously described as an episome capable of inducing differentiation upon transfection into a dedifferentiated rat hepatoma cell line, was mapped on human chromosome 12q24.2-12q24.3. This chromosomal location was indistinguishable by in situ hybridization from that of the gene coding for the hepatic transcription factor HNF1. The sequence of the integrated form of the episome as well as its flanking sequences show that it is rich in retroposons. It contains a human ribosomal protein L21 processed pseudogene, one truncated L1Hs sequence, and 10 Alu repeats, which belong to different subfamilies.

• OSTI ID: 6758613
• Journal Information: Somatic Cell and Molecular Genetics; (United States), Vol. 20:3; ISSN 0740-7750
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 12
GENETIC MAPPING
LIVER CELLS
CELL DIFFERENTIATION
DNA HYBRIDIZATION
DNA SEQUENCING
ANIMAL CELLS
BIOTECHNOLOGY
CHROMOSOMES
GENETIC ENGINEERING
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID HYBRIDIZATION
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics