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Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter

Journal Article · · Genomics; (United States)
; ;  [1]; ;  [2];  [3]
  1. Univ. of California, San Diego, CA (United States)
  2. Univ. of California, Irvine, CA (United States)
  3. Salk Institute for Biological Studies, San Diego, CA (United States)

Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter. 17 refs., 2 figs.

OSTI ID:
6758364
Journal Information:
Genomics; (United States), Journal Name: Genomics; (United States) Vol. 14:4; ISSN GNMCEP; ISSN 0888-7543
Country of Publication:
United States
Language:
English

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