The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD[sub 67] and GAD[sub 65]) suggests that they derive from a common ancestral GAD

Journal Article · Sun May 01 04:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1246· OSTI ID:6758241

Bu, D F; Tobin, A J ^[1]

---

1. Univ. of California, Los Angeles, CA (United States)

The authors have cloned and characterized human genes (GAD1 and GAD2) encoding the two human glutamate decarboxylases, GAD[sub 67] and GAD[sub 65]. The coding region of the GAD[sub 65] gene consists of 16 exons, spanning more than 79 kb of genomic DNA. Exon 1 contains the 5[prime] untranslated region of GAD[sub 65] mRNA, and exon 16 specifies the protein's carboxy terminal and at least part of the mRNA's 3[prime] untranslated sequence. Similarly, the coding region of the GAD[sub 67] gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD[sub 67] gene contains an additional exon (exon 0) that, together with part of exon 1, specifies the 5[prime] untranslated region of GAD[sub 67] mRNA. Exon 16 specifies the entire 3[prime] untranslated region of GAD[sub 67] mRNA. Exons 1-3 encode the most divergent region of GAD[sub 65] and GAD[sub 67]. The remaining exon-intron boundaries occur at identical positions in the two cDNAs, suggesting that they derive from a common ancestral GAD gene. 26 refs., 4 figs., 2 tabs.

• OSTI ID: 6758241
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:1; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CARBON-CARBON LYASES
CARBOXY-LYASES
CLONING
DECARBOXYLASES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENES
GENETIC ENGINEERING
HYBRIDIZATION
LYASES
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS