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Title: Calibration method for spectroscopic systems

Abstract

Calibration spots of optically-characterized material placed in the field of view of a spectroscopic system allow calibration of the spectroscopic system. Response from the calibration spots is measured and used to calibrate for varying spectroscopic system operating parameters. The accurate calibration achieved allows quantitative spectroscopic analysis of responses taken at different times, different excitation conditions, and of different targets. 3 figs.

Inventors:
Publication Date:
Research Org.:
Sandia Corporation
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
675780
Patent Number(s):
US 5,838,435/A/
Application Number:
PAN: 8-953,814
Assignee:
Sandia Corp., Albuquerque, NM (United States) SNL; SCA: 400102; PA: EDB-99:002757; SN: 98002027943
DOE Contract Number:
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 17 Nov 1998
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; SPECTROMETERS; CALIBRATION STANDARDS; OPTICAL PROPERTIES; QUANTITATIVE CHEMICAL ANALYSIS

Citation Formats

Sandison, D.R.. Calibration method for spectroscopic systems. United States: N. p., 1998. Web.
Sandison, D.R.. Calibration method for spectroscopic systems. United States.
Sandison, D.R.. 1998. "Calibration method for spectroscopic systems". United States. doi:.
@article{osti_675780,
title = {Calibration method for spectroscopic systems},
author = {Sandison, D.R.},
abstractNote = {Calibration spots of optically-characterized material placed in the field of view of a spectroscopic system allow calibration of the spectroscopic system. Response from the calibration spots is measured and used to calibrate for varying spectroscopic system operating parameters. The accurate calibration achieved allows quantitative spectroscopic analysis of responses taken at different times, different excitation conditions, and of different targets. 3 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1998,
month =
}
  • Calibration spots of optically-characterized material placed in the field of view of a spectroscopic system allow calibration of the spectroscopic system. Response from the calibration spots is measured and used to calibrate for varying spectroscopic system operating parameters. The accurate calibration achieved allows quantitative spectroscopic analysis of responses taken at different times, different excitation conditions, and of different targets.
  • An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which maymore » then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.« less
  • The present invention relates to a method for use in liquid scintillation measurements to feed an accurately determined amount of radioactive substance to a fluid scintillation system for a calibration measurement. According to the invention an accurately determined amount of radioactive substance is adsorbed to a carrier, which is introduced into the fluid scintillation system. The invention also relates to a carrier for use in the method.
  • A purged window apparatus is described which utilizes tangentially injected heated purge gases in the vicinity of electromagnetic radiation transmitting windows and a tapered external mounting tube to accelerate these gases to provide a vortex flow on the window surface and a turbulent flow throughout the mounting tube thereby preventing backstreaming of flowing gases under investigation in a chamber to which a plurality of similar purged apparatus is attached with the consequent result that spectroscopic analyses can be undertaken for lengthy periods without the necessity of interrupting the flow for cleaning or replacing the windows due to contamination.