Differentiation of normal and cultured preneoplastic tracheal epithellal cells in rats: importance of epithelial mesenchymal interactions

Journal Article · Sat Nov 01 04:00:00 EST 1980 · J. Natl. Cancer Inst.; (United States)

OSTI ID:6757003

Terzaghi, M; Klein-Szanto, A J.P.

Changes in the dependence on mesenchymal tissues for survival and differentiation in inbred F344 female rats were investigated in tracheal epithelial cells exposed to 7,12-dimethylbenz(a)anthracene (DMBA) and 12-O-tetradecanoylphorbol 13-acetate (TPA). Fresh suspensions of normal tracheal epithelium or cultured preneoplastic cells were inoculated into isolated organ segments (trachea, esophagus, bladder, or small intestine) or into Dacron containers that were then implanted subdermally into isogenic recipients. At various times after cell inoculation and implantation, tissues were removed for histologic evaluation. Normal cells inoculated into frozen-thawed trachea, esophagus, bladder, and intestine yielded a regular mucociliary epithelium. Normal cell inocula did not, however, survive in trachea previously heated (100/sup 0/C), fixed in ethanol, or digested with collagenese; nor did normal cells survive in Dacron containers unless tracheal fibroblasts plus epithelial cells were inoculated together. DMBA- and TPA-exposed cell populations with increased growth capacity in vitro survived and differentiated on all of the above substrates. For survival and differentiation in vivo, preneoplastic cells appeared to have less stringent substrate requirements than did normal cells. Application of the described techniques to the study of changes occurring early in the development of neoplastic disease is discussed.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6757003
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 65:5; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BLADDER
BODY
CARCINOGENS
CELL CULTURES
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DACRON
DIGESTIVE SYSTEM
DIMETHYLBENZANTHRACENE
EPITHELIUM
ESOPHAGUS
ESTERS
FIBROBLASTS
GASTROINTESTINAL TRACT
INTESTINES
MAMMALS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
PHORBOL ESTERS
POLYESTERS
POLYMERS
RATS
RESPIRATORY SYSTEM
RODENTS
SMALL INTESTINE
SOMATIC CELLS
TISSUES
TRACHEA
URINARY TRACT
VERTEBRATES