c-Type cytochromes and manganese oxidation in Pseudomonas putida MnB1
- Univ. of California, San Diego, La Jolla, CA (United States). Scripps Institution of Oceanography
Pseudomonas putida MnB1 is an isolate from an Mn oxide-encrusted pipeline that can oxidize Mn(II) to Mn oxides. The authors used transposon mutagenesis to construct mutants of strain MnB1 that are unable to oxidize manganese, and they characterized some of these mutants. The mutants were divided into three groups: mutants defective in the biogenesis of c-type cytochromes, mutants defective in genes that encode key enzymes of the tricarboxylic acid cycle, and mutants defective in the biosynthesis of tryptophan. The mutants in the first two groups were cytochrome c oxidase negative and did not contain c-type cytochromes. Mn(II) oxidation capability could be recovered in a c-type cytochrome biogenesis-defective mutant by complementation of the mutation.
- OSTI ID:
- 675501
- Journal Information:
- Applied and Environmental Microbiology, Vol. 64, Issue 10; Other Information: PBD: Oct 1998
- Country of Publication:
- United States
- Language:
- English
Similar Records
cumA, a gene encoding a multicopper oxidase, is involved in Mn{sup 2+} oxidation in Pseudomonas putida GB-1
Expression, crystallization and preliminary diffraction studies of the Pseudomonas putida cytochrome P450cam operon repressor CamR