Changes in metallothionein mRNA and protein after sublethal exposure to arsenite and cadmium chloride in juvenile winter flounder

Journal Article · Thu Jan 01 04:00:00 EST 1998 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1002/etc.5620170517· OSTI ID:675488

Jessen-Eller, K ^[1]; Crivello, J F ^[2]

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1. Woods Hole Oceanographic Inst., MA (United States)
2. Univ. of Connecticut, Storrs, CT (United States). Dept. of Physiology and Neurobiology

Juvenile winter flounder (Pleuronectes americanus) from a Long Island Sound site within the Niantic River region contain elevated levels of liver As and Cd. Previous work in mammals suggests that a reduced species of As, NaAs{sup 3+}, is as potent a metallothionein (MT) inducer as CdCl{sub 2}. The authors investigated whether this is also the case in winter flounder by subcutaneously exposing juveniles to a sublethal range of NaAs{sup 3+} (0, 0.38, 0.75, and 1.5 {micro}mol/g fish) or CdCl{sub 2} (0, 0.045, 0.09, and 0.18 {micro}mol/g). Both metals rapidly induced MT protein within 24 h of exposure, but only the highest dose of CdCl{sub 2} elicited a significant increase in MT mRNA. The magnitude of the MT protein response to CdCl{sub 2} (7.25-fold) demonstrates that elevated liver As and Cd do not narrow the MT response window in juvenile flounder from the Niantic River region. These fish have relatively low liver MT protein levels (46 {micro}g/g) and will be a useful reference population for future metal studies within Long Island Sound.

• Sponsoring Organization: USDOE
• OSTI ID: 675488
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 1 Vol. 17; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ARSENIC
BIOLOGICAL INDICATORS
BIOLOGICAL MARKERS
CADMIUM
FISHES
MESSENGER-RNA
METALLOTHIONEIN
WATER POLLUTION