New pathway for the biodegradation of indole in Aspergillus niger

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6749943

Kamath, A; Vaidyanathan, C S ^[1]

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1. Indiana Institute of Science, Bangalore (India)

Indole and its derivatives form a class of toxic recalcitrant environmental pollutants. The growth of Aspergillus niger was inhibited by very low concentrations (0.005 to 0.02%) of indole, even when 125- to 500-fold excess glucose was present in the medium. When 0.02% indole was added, the fungus showed a lag phase for about 30 h and the uptake of glucose was inhibited. Indole was metabolized by a new pathway via indoxyl (3-hydroxyindole), N-formylanthranilic acid, anthranilic acid, 2,3-dihydroxybenzoic acid, and catechol, which was further degraded by an ortho cleavage. The enzymes N-formylanthranilate deformylase, anthranilate hydroxylase, 2,3-dihydroxybenzoate decarboxylase, and catechol dioxygenase were induced by indole as early as after 5 h of growth, and their activities were demonstrated in a cell-free system.

• OSTI ID: 6749943
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:1; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
ASPERGILLUS
AZAARENES
AZOLES
BIODEGRADATION
BIOLOGICAL PATHWAYS
CARBON-CARBON LYASES
CARBOXY-LYASES
CHEMICAL REACTIONS
DECARBOXYLASES
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
EUMYCOTA
FUNGI
HETEROCYCLIC COMPOUNDS
HYDROXYLASES
INDOLES
LYASES
METABOLISM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PLANTS
PYRROLES